De novo design and generation of recombinant enzymes for enantiospecific conversion of 2-oxoadipate to (R)-2-hydroxyadipate

Adipic acid is among the most-produced chemicals worldwide, with approximately 2.5 billion kilograms synthesized annually, and a global market of over $8 billion. the most notable use for adipic acid is for the syntehsis of nylon, used in upholstery, auto parts, apparel, and other products. Standard industrial methods for adipic acid synthesis are costly and have major drawbacks, including consumption of fossil fuels, inefficient yields, and the production of greenhouse gases. This invention is an enzyme, and the nucleic acid sequences to encode the enzyme, that can be used to produce adipic acid and other compounds in bacteria and other microorganisms. This bio-based method is less expensive and has fewer environmental impacts than current methods of producing adipic acid and other industrial chemicals.

Duke File (IDF) Number



  • Reitman, Zachary
  • Choi, Bryan
  • Sampson, John
  • Yan, Hai

For more information please contact


School of Medicine (SOM)