Methods for identifying compounds that regulate beta-arrestin signaling complexes
Researchers at Duke University have developed a a method to detect the formation of a specific -arrestin-biased signaling complex. After treatment with test compounds, murine brain striata were analyzed for the formation of a -arrestin-based signaling complex associated with dopamine receptor and chemokine receptor CXCR4 stimulation. Using this method, a compound that could block the formation of the -arrestin complex compound but had no effect on G-protein mediated signaling was identified. Further analysis demonstrated this compound has no effect in -arrestin-deficient mice. This assay can be applied to identify other compounds capable of blocking the formation of this -arrestin-based signaling to develop a new class of GPCR drugs that specifically target the alternative GPCR signaling pathway.
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