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Redox Maps of Disease

Value Proposition

Protein-protein interactions (PPIs) are essential for normal biological functions and many human diseases are caused by, or can lead to the dysfunction of these interactions. Identifying PPIs plays a crucial role in the modern drug discovery process: more than 80% of current pharmaceutical drugs act through their effect on protein interactions. Prior biochemical and genetic analyses of PPIs have not given due importance to the presence of reactive oxygen species (ROS) and reactive nitrogen species (RNS) on PPIs. However, ROS/RNS can modulate PPIs and are widely implicated in disease pathogenesis. Diseases such as Alzheimer’s disease, atherosclerosis, Parkinson’s disease, stroke, aging ARDS, asthma, have elevated ROS/RNS levels. In contrast, cancer cells and infectious microbes have a high tolerance for ROS/RNS. Therefore, understanding redox-state dependent PPIs can reveal disease mechanisms and uncover novel therapeutic targets.

Technology

This technology is a large-scale high throughput proteomics screen for discovering redox dependent PPIs. Protein of interest can be entered into the proteomic screen to identify binding to proteins, DNA, RNA and peptides under various redox conditions. Protein interactions revealed through this method can provide insights into disease mechanisms as well as help uncover novel therapeutic targets. In addition, newly identified interacting partners can be entered into the proteomic screen to further expand the redox map of disease.

Advantages

  • High throughput platform for redox-dependent interaction proteomics
  • Suitable for large scale screening
  • Cost effective

Duke File (IDF) Number

T-002173

Inventor(s)

  • Stamler, Jonathan
  • Liu, Limin
  • Okado-Matsumoto, Ayako

Publications

  • Matsumoto A, Comatas KE, Liu L, Stamler JS. “Screening for Nitric Oxide-Dependent Protein-Protein Interactions” Science. 2003 Aug 301(5633): 657-661.

For more information please contact

College

School of Medicine (SOM)

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