Solid-Phase Alkyl Aryl Mixed Disulfides for Highly Efficient Cysteine/Thiol Immobilization

Technology

The inventors have developed a strategy for purification of post-translationally modified peptides or proteins, and the identification of the various modifications. The methodology involves solid-phase immobilization of cysteine/thiol groups using mixed disulfides of resin-bound alkyl and aryl thiols. The method was utilized for the analysis of numerous cellular cysteine-modification including S-nitrosylation and S-palmitoylation.

Advantages

  • Efficient solid-phase immobilization of cysteine residues
  • Considerably more reactive than other commercially available resins

Duke File (IDF) Number

T-002752

Inventor(s)

  • Stamler, Jonathan
  • Forrester, Michael
  • Foster, Matthew

Patents

    • Patent Number: 9,086,411
    • Title: RESIN ASSISTED CAPTURE OF CYSTEINE-MODIFIED PROTEINS/PEPTIDES AND DETERMINATION OF PRESENCE AND LOCATION OF MODIFICATION
    • Country: United States of America

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College

School of Medicine (SOM)